Vol. 7, Issue 1, Part A (2025)

Medicinal plants contain many active substances that act as antioxidants as part of the requirements of sustainable development and environmentally friendly green farming technologies. This study was conducted at Al-Jaflawi Poultry Hatchery from November 22, 2024 to November 25, 2024 to study the effect of injecting hatching eggs with aqueous extract of annatto seeds on some biochemical characteristics of broiler chicks exposed to post-hatching nutritional fasting. 300 fertilized eggs were used divided to four groups, each group 75 eggs, and injected with aqueous extract of annatto seeds at 18 days of embryo age. The groups were G1 is free, G2, G3, and G4 In ovo injected with 0.1, 0.2, and 0.3 ml aqueous extract of annatto seeds / egg in amniotic sac. The results were: significant decrease for G2, G3, and G4 groups compared to G1 group in cholesterol at 12 hours, significant decrease for G2, G3, and G4 groups on G1 group in triglyceride at 12 hours, significant decrease for G3, and G4 groups on G1 group in glucose at hatching and 12 hours, significant improvement in Glutathione peroxidase (GSH-PX) for In ovo injection groups compared to G1 group.